
<艾美捷科技代理Bold Biotechnology(BBT)品牌全系列产品
Bold Biotechnology Co., Ltd (简称BBT)是一家综合性健康产业集团,拥有卓越的生命健康技术。BBT致力于研发全球最前沿的领先技术,涵盖以下领域:癌症预防、免疫细胞治疗(BAK、NK、DC免疫疗法)、外泌体研发、血清无免疫细胞用干细胞培养基、超早期癌症筛查、发酵马乳外泌体研发与生产,高浓度维他命C制备等十余项核心生物技术。BBT公司的主要包括细胞培养基相关产品以及免疫化学检测类相关产品。

▍BBT公司产品线
● αβ-T・γδ-T细胞培养基及配套产品
EALI515-T 无血清培养基:专用于人类及小鼠外周血淋巴细胞的培养。该培养基支持多种淋巴细胞的增殖与活化(包括αβ-T细胞、γδ-T细胞、CTL细胞、NKT细胞及NK细胞),适用于癌症免疫疗法。
| Product | Description | Code | Package | Store at |
| EALITM515-T (Without IL-2) | Originally modified medium based on Iscove’s modified Dulbecco’s Medium (IMDM). Basal medium for low-serum or serum-free culture of human and mouse Lymphoblast cells. pH: 7.1-7.6 Osmolarity: 280-300 mOsm/Kg H2O Membrane filtered, Endotoxin tested, Cell culture teste | 1502 | 1,000 mL | 2~10℃ |
● NK细胞培养基
EALI515-NK 无血清培养基:专用于人类自然杀伤细胞(NK细胞)培养。本培养基支持NK细胞的活化与增殖,适用于癌症免疫疗法。
<| Product | Description | Code | Package | Store at |
| EALITM515-NK (Without IL-2) | Basal medium for low-serum or serum-free culture of human and mouse Lymphoblast & NK cells. pH: 7.1-7.6 Osmolarity: 280-300 mOsm/Kg H2O Membrane filtered, Endotoxin tested, Cell culture tested. | 1602 | 1,000 mL | 2~10℃ |
| EALITM515-NK (IL-2 700 IU/mL) | Recommend for expansion culture of NK cells. Containing 700 IU/mL of IL-2. pH: 7.1-7.6 Osmolarity: 280-300 mOsm/Kg H2O Membrane filtered, Endotoxin tested, Cell culture tested. | 1622 | 1,000 mL | 2~10℃ |
| EALITM515-NK (IL-2 1,000 IU/mL) | Recommend for early stage culture of NK cells. Containing 1,000 IU/mL of IL-2. pH: 7.1-7.6 Osmolarity: 280-300 mOsm/Kg H2O Membrane filtered, Endotoxin tested, Cell culture tested | 1632 | 1,000 mL | 2~10℃ |
● 间充质干细胞(MSCs)无血清/无异源培养基及配套产品
αMSCM 培养基:基于改良型αMEM基础培养基优化开发,支持牙髓、脐带、骨髓及脂肪组织来源间充质干细胞(MSCs)的无异源/低血清培养。
<| Product | Description | Code | Package | Store at |
| Basal medium (modified αMEM) αMSCM Basal | A basal medium without L-Glutamine and Cell Growth Factors (GFs). (containing rh-Transferrin and rh-Insulin). This product can be used for low serum culture of various MSCs. Add 2mM L-Glutamine, Cell growth Factors and 2% Fetus Bovine Serum (FBS) before use. Recommend use of x100 3GF Supplement (code: 3151F) as additive. | 1801 | 500 mL | 2~10℃ |
| Xeno-Free culture medium αMSCM+PL | This Product is a set of Xeno-Free medium and human Platelet Lysate (hPL:UltraGro*). No need additional growth supplements, serum and cell adhesion molecule. | 1811 | 500 mL | 2~10℃ |
| Cell Growth factor mixture x100 3GF Supplement | A mixture of recombinant human FGF-2, PDGF-BB and TGF-β1. Contains recombinant human albumin as a stabilizer.This product is lyophilized. | 3151F | 5 mg | 4~8°C |
● 蛋白质生产用化学成分确定培养基
EALI515-MAb 培养基:杂交瘤细胞培养与抗体生产专用培养基,本产品采用无动物源性蛋白配方(不含血清白蛋白),能支持多种杂交瘤细胞的高效增殖并维持抗体生产能力。培养基中仅含胰岛素与转铁蛋白两种蛋白组分,相比含血清或白蛋白的培养基,可显著简化培养上清中单克隆抗体的分离纯化流程。
<| Product | Description | Code | Package | Store at |
| EALI 515-MAb | Newly designed chemically defined medium of Hybridoma cell culture and production of Monoclonal Antibody. Containing hr-Insulin and hr-Transferrin. Without Serum Albumin or other animal derived proteins. pH: 7.3-7.7 Osmolarity: 280-300 mOsm/Kg H2O Membrane filtered, Endotoxin tested, Cell culture tested. | 1402 | 1,000 mL | 2~10℃ |
● 其他细胞培养相关产品
| Product | Description | Code | Package | Store at |
| Dulbecco’s Phosphate Buffered Saline, “PBS(-)” | Dulbecco’s Phosphate–Buffered Saline pH: 7.3-7.5 Osmolarity: 276-284 mOsm/Kg H2O Membrane filtered Endotoxin tested | 2101 | 500 mL | Room temp. |
| Anti CD3 MAb for CIK TactimabTM | Affinity purified anti-CD3 monoclonal antibody without preservative. Manufactured by animal protein free culture of mouse Hybridoma clone OKT3 cell. Stimulate the growth of human T Lymphocyte in vitro. Membrane filtered. Endotoxin tested. T cell culture tested. This product is lyophilized. | 3121F | 1 ㎎ | Freezing |
| Anti CD3 MAb for CIK TactimabTM | Affinity purified anti-CD3 monoclonal antibody without preservative. Manufactured by animal protein free culture of mouse Hybridoma clone OKT3 cell. Stimulate the growth of human T Lymphocyte in vitro. Membrane filtered. Endotoxin tested. T cell culture tested. This product is lyophilized. | 3122F | 10 ㎎ | Freezing |
● 免疫化学检测试剂盒与试剂
| Product | Description | Code | Package |
| CTX-ELISA 1B | Plate Wash Buffer x20 conc, W-1 | 8111 | 1 x 50 mL |
| CTX-ELISA 1B | STD/Sample Diluent, D-1 | 8112 | 1 x 50 mL |
| CTX-ELISA 1B | Anti-CTX1B-ALP Diluent, D-2 | 8113 | 1 x 50 mL |
| CTX-ELISA 1B | ALP Substrate Solution (pNPP), R-1 | 8114 | 1 x 50 mL |
| CTX-ELISA 1B | ELISA Assay Plate CTX1B | 8115 | 2 x 96 well |
| CTX-ELISA 1B | Anti-CTX1B-ALP:Freeze dry powder | 8116 | 1 tube |
| CTX-ELISA 1B | CTX-1B STD 5 ng/mL | 8117 | 1 x 100 μL |
| CTX-ELISA 1B | Adhesive plastic sheet | -- | 6 sheet |
| CTX-ELISA 1B | Operation manual | -- | 1 |
| Plate Wash Buffer x20 conc. W-1 | x20 concentrated Tris-buffer(pH 7.5) with tween 20, blocking reagent and preservative. 20 hold dilute with pure water before use. | 8111 | 50 mL |
| STD/Sample Diluent D-1 | Specially developed buffer solution to dissolve hydrophobic Ciguatoxin molecule into the water. pH 7.5.Ready to use. | 8112 | 50 mL |
| Anti-CTX1B-ALP Diluent D-2 | Specially designed buffer solution(pH 7.5) for dilution of alkaline-phosphatase conjugated antibody, with Mg++, blocking material and detergent. Ready to use. | 8113 | 50 mL |
| ALP Substrate Solution (pNPP) R-1 | Alkaline-phosphatase substrate solution. Alkaline solution of 0.01% disodium p-Nitrophenylphosphate (pNPP). pH10,Useful for colorimetric assay of Alkaline-phosphatase. Ready to use. | 8114 | 50 mL |
| ELISA Assay Plate CTX1B | Anti-CTX1B antibody immobilized 96 well micro-plate. Protected with blocking reagents to stabilize antibodies. More than 8 months store is possible under dry conditions of 4 ℃ or less. | 8115 | 1 plate |

艾美捷科技代理Gene Tools品牌
Gene Tools是一家生产和销售Morpholino Anti-sense oligos(反义寡核苷酸)的公司,其产品专门用于基因调控的科学研究和药物开发。Gene Tools公司由多位资深科学家组成,为全球客户提供免费的技术咨询和Morpholino 序列设计服务。 Morpholino作为基因调控研究的最佳工具,已被广泛用于:阻断靶mRNA 翻译 (下调基因);诱导RNA选择性剪接 (下调或上调基因);阻断 miRNA;阻断病毒 RNA;阻断 non-coding RNA。
▍Morpholino介绍
Morpholino属于第三代反义寡核苷酸,主要通过阻断蛋白质翻译过程而达到抑制目标基因功能的作用,目前已经广泛用于发育生物学和细胞学实验中,并取得了非常好的实验效果。
Morpholino以吗啉环类似物为基本骨架,这种独特的结构特性使得Morpholino 具有以下几个特点:
● 与其他类型的反义寡核苷酸相比,Morpholino 能更好地抵抗核酸酶的作用;
● Morpholino 与靶序列结合后通过空间位阻效应发挥作用, 不激活RNaseH,不引起目标基因mRNA 的降解 ;
● 针对翻译起始位点区设计的Morpholino 用于抑制蛋白质的翻译,针对 pre-mRNA 剪切位点区设计的Morpholino 可影响mRNA 剪切,得到不 同的转录本,可以方便地用于目标基因结构域的突变研究;
● 与靶序列结合能力强,可以渗入mRNA 的二级结构, 并且特异性好。
配合Morpholino 的使用,Gene Tools公司开发了相应的递送系统,可以安全、高效地将Morpholino 输送至胞内,因此,Morpholino 也可以用于遗传学研究和新药靶标的发现以及验证。此外,Morpholino 可以标上荧光基团、亲和标签以及活性反应基团,以供不同的研究需要。

▍Gene Tools公司产品介绍
● Custom Morpholino:可以根据目标基因序列定制设计并合成Morpholino,用于体外细胞实验或某些直接显微注射的模型(如斑马鱼、非洲爪蟾胚胎)。
● Vivo-Morpholino:适用于活体动物模型(小鼠,大鼠,狗,灵长类等),细胞培养,器官培养等。通过特殊的末端修饰,提高了其穿透细胞膜进入组织细胞的能力,使得在成年动物(主要是小鼠、大鼠)中进行系统性基因功能研究成为可能。
● Prepared Control Oligos:根据特定需求合成细胞穿透肽偶联的 Morpholino。适用于活体动物模型(小鼠,大鼠,狗,灵长类等),细胞培养,器官培养等。
| 产品名称 | Short Description | Oligo sequence | SKU |
| Standard Control Oligo | Negative control oligo | CCTCTTACCTCAGTTACAATTTATA | PCO-StandardControl-100 |
| Vivo-Morpholino Standard Control Oligo | Negative Vivo-Morpholino control oligo | CCTCTTACCTCAGTTACAATTTATA | PCO-VivoStandardControl-100 |
| Random Control Oligo 25-N | 25-base random sequence mixture | NNNNNNNNNNNNNNNNNNNNNNNNN | PCO-RandomControl-25N-100 |
| Zebrafish p53 oligo | Danio rerio apoptosis suppression oligo | GCGCCATTGCTTTGCAAGAATTG | PCO-ZebrafishP53-100 |
| Zebrafish Chordin Positive Control Oligo | Danio rerio Chordin positive control oligo | ATCCACAGCAGCCCCTCCATCATCC | PCO-ZebrafishChordin-100-F |
| Clawed Frog Beta Catenin Positive Control Oligo | Xenopus laevis Beta Catenin antisense oligo | TTTCAACCGTTTCCAAAGAACCAGG | PCO-BetaCatenin-100-F |
| Green Fluorescent Protein Positive Control | Translation blocker for GFP target | ACAGCTCCTCGCCCTTGCTCACCAT | PCO-GFPControl-100 |
| Vivo-Morpholino GFP Positive Control | Translation blocker for GFP target (Vivo-Morpholino) | ACAGCTCCTCGCCCTTGCTCACCAT | PCO-VivoGFPControl-100 |
| Fluoresceinated Vivo-Morpholino Std Control | Negative Vivo-Morpholino control oligo with 5'Fluorescein | CCTCTTACCTCAGTTACAATTTATA | PCO-FluorVivoStandardControl-100-F |
● Endo-Porter:为了解决Morpholino在体外培养细胞中摄取效率低的问题,Gene Tools 开发了 Endo-Porter 这种特殊的转染试剂。它通过温和的内吞体逃逸机制帮助Morpholino进入细胞质。